Please read Metabolite Processing Precautions before proceeding.
⦁ Prepare and separate all material and solutions before you start. Check the Recommended Resources List.
⦁ Allow the samples thaw in ice.
⦁ While samples are thawing, prepare one tube labeled as QC (pooled quality control). : In this tube you will put together one aliquot of all your samples (we recommend 10% of the initial sample volume). For that, make sure to pick a tube able to fit the final volume capacity.
⦁ Centrifuge samples at 10,000 g for 10 min at 4°C to remove particulates.
⦁ Transfer 50 μL to a labeled maximum recovery vial containing 100 μL extraction solution B and mix well.
⦁ For the QC sample, transfer 5 μL of each sample to the QC tube.
⦁ Add extraction solution B (1:2 sample vol:Sol B vol) and vortex. Ex. For 30 urine samples, the final volume will be 150 μL. So, the volume of sol B to be added will be 300 μL. PAUSE POINT: Store the samples at -20°C for up to 12 weeks.
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