⦁ Please be Careful when handling diseased samples.
⦁ Take care not to generate aerosols and ALWAYS wear protective clothing (lab coat, mask, googles, and gloves).
Blood Collection Procedures
⦁ Devices used for blood collection are always a source of contaminants. We suggest purchasing enough
of these important consumables to last for the duration of the study.
- The choice of an anticoagulant for metabolomics applications is still a subject of discussion
and there is a lack of clear recommendations. Based on current evidence, we recommended EDTA (ethylenediaminetetraacetic acid) or Heparin as anticoagulant over sodium citrate.
- Fill 3 sampling devices with ultrapure water and label as Blank 1, 2 and 3. These will be treated identically as your samples.
⦁ Avoid hemolyzed samples by placing whole blood immediately in ice cold water after collection.
- Keep samples on ice (or at around 4˚C) until all samples are finished.
⦁ Transfer 500 µL aliquots of blood (take at least 2 from each subject in order to have a backup sample
for any potential repeat analyses) into new 2 mL labelled tubes (DO NOT USE COLORED TUBES) using a
pipette with an appropriate filter tip.
- Stored the samples at -80°C until analyzed and, if needed, all transportation should be carried out in dry ice.
PLEASE FOLLOW RULES FOR
LABELLING.