⦁ Please be Careful when handling diseased samples.
⦁ Take care not to generate aerosols and ALWAYS wear protective clothing (lab coat, mask, googles, and gloves).
⦁ Devices used for blood collection are always a source of contaminants. We suggest purchasing sufficient quantities
of these important consumables to last for the duration of the study.
Serum Obtenion Procedures
⦁ Fill 3 devices with ultrapure water and label as Blank 1, Blank 2 and Blank 3. These
will be treated the same way as your samples.
⦁ After blood collection,
let the tubes sit upright at room temperature for a minimum of 30 min to a maximum of 60 min to allow for clot formation.
⦁ After clot formation, centrifuge the samples at 1500x g at 4°C for 20 min.
⦁ Immediately transfer 500 µL aliquots of serum (make at least 2 from each subject in order to have a backup
sample for any potential repeat analyses) into new 2 mL labelled tubes (DO NOT USE COLORED TUBES) using a
pipette with an appropriate filter tip (check options in the Resources list).
⦁ Store the samples at -80°C until analyzed and, if needed, all transportation should be carried out in dry ice.
PLEASE FOLLOW THE RULES FOR LABELLING.